CrISPR mix, MIMIC and RMCE Microinjection

General information

• For DNA plasmid preparation recommendation, see here.

• Injection of 400 embryos

• Three options are available

  • Injection only: we inject and send out G0 larvae. Screening for the transformants must be done by the customer. No re-injection service is offer if no transformant line is obtained.

  • Injection and screening for transformants: we inject, cross G0 survivers and screen for transformants. Standard injection service include a visible marker screening (w+ or y+). If necessary, a second microinjection is included. Positive lines are crossed and sent. We normally send between 1-4 transformants. Balancing must be done by the customer.

  • Injection and balancing: we inject, cross G0 survivers, screen for transformants and balance the lines. Standard injection service include a visible marker screening (w+ or y+). If necessary, a second microinjection is included. Positive lines will be balanced to appropriate balancers and Cas9 removed. We guarantee at least 1-4 lines.

• Avalaible CRISPR mix injection fly stock:

  • y[1] M{w[+mC]=nos-Cas9.P}ZH-2A w[*] (X) (ready to inject)

  • w[1118]; PBac{y[+mDint2]=vas-Cas9}VK00027 (3rd) (ready to inject)

• Other stocks may be available for microinjection or you can send your stock for embryo collection. If you chose the latter option, an additional 3-4 weeks will be necessary for the service to amplify new stocks. (55$ is applicable for each stock).

• There is additional charge for fluorescent or vermilion marker screening (see price list).

• Our aim is to help you with your experimental design as much as we can and to keep the transforming process as simple as possible for you. If you have any questions or comments, send us an email.