PhiC31 plasmid microinjection

General information

For DNA plasmid preparation recommendation, see here.
Injection of 300 embryos
Three options are available
- Injection only: we inject and send out G0 larvae. Screening for the transformants must be done by the customer. No re-injection service is offer if no transformant line is obtained.
- Injection and screening for transformants: we inject, cross G0 survivers and screen for transformants. Standard injection service include a visible marker screening (w+ or y+). If necessary, a second microinjection is included. Positive lines are crossed and sent. We normally send between 1-4 transformants. Balancing must be done by the customer.
- Injection and balancing: we inject, cross G0 survivers, screen for transformants and balance the lines. Standard injection service include a visible marker screening (w+ or y+). If necessary, a second microinjection is included. Positive lines will be balanced to appropriate balancers and integrase removed. We guarantee at least 1-4 lines for plasmid at standard size (<25 kb).
For larger construct (>25 kb), we suggest to order the large plasmid size service to insure an appropriate number of transformants. We will inject 600 embryos.
There is additional charge for fluorescent or vermilion marker screening (see price list).
Avalaible injection PhiC31 fly stock:
- y[1] w[*] P{y[+t7.7]=nos-phiC31\int.NLS}X ; P{y[+t7.7]=CaryP}attP40 (2L) (ready to inject)
- y[1] M{vas-int.Dm}ZH-2A w[*]; M{attP'} ZH-51C (-RFP) (2R) (ready to inject)
- y[1] w[*] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP} attP2 (3L) (ready to inject)
- y[1] M{vas-int.Dm}ZH-2A w[*]; M{attP} ZH-86Fb (-RFP) (3R) (ready to inject)
- y[1] M{vas-int.Dm}ZH-2A w[*]; PBac{y[+]-attP-3B}VK00033 (3L) (ready to inject)
- y[1] M{vas-int.Dm}ZH-2A w[*]; PBac{y[+]-attP-3B}VK00037 (2L) (ready to inject)
Other stocks may be available for microinjection or you can send your stock for embryo collection. If you chose the latter option, an additional 3-4 weeks will be necessary for the service to amplify new stocks. (55$ is applicable for each stock).
Our aim is to help you with your experimental design as much as we can and to keep the transforming process as simple as possible for you. If you have any questions or comments, send us an email.

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PhiC31 plasmid microinjection

General information

For DNA plasmid preparation recommendation, see here.

Injection of 300 embryos

Three options are available

For larger construct (>25 kb), we suggest to order the large plasmid size service to insure an appropriate number of transformants. We will inject 600 embryos.

There is additional charge for fluorescent or vermilion marker screening (see price list).

Avalaible injection PhiC31 fly stock:

Other stocks may be available for microinjection or you can send your stock for embryo collection. If you chose the latter option, an additional 3-4 weeks will be necessary for the service to amplify new stocks. (55$ is applicable for each stock).

Our aim is to help you with your experimental design as much as we can and to keep the transforming process as simple as possible for you. If you have any questions or comments, send us an email.

Our services are for research use only.